Abstract


GENETICS OF ADAPTATION IN A TEMPERATE SEA ANEMONE: SEARCH FOR SELECTION AMONG ECOLOGICALLY AND MORPHOLOGICALLY DIFFERENTIATED POPULATIONS USING NGS DATA

Anemonia viridis is a symbiotic temperate species commonly found from the English Channel to the Mediterranean Sea from shallow water down to 30m deep as well as in lagoons. Five different morphs have been described in this species based on the expression profiles of GFP-like proteins. Two ecologically contrasted populations (shallow vs deep, open sea vs lagoon) were sampled from 5 locations, and individuals from three morphs were sampled in several locations from the English Channel and the Mediterranean Sea. In order to understand the genetic basics of adaptation to differing temperature regimes, we are scanning the genome of over nearly 400 individuals for signature of selection. We looked for Fst outliers from a pool dataset of RAD and 12 EPIC markers. Each EPIC marker targets a different gene whose expression is modified under a temperature stress, as revealed in our lab by a transcriptomic approach (Ganot et al. 2011, Moya et al. 2012). Based on a subset of individuals, preliminary results did not show any clear genetic differentiation among the morphs. Through the analysis of the whole dataset, we will answer the following questions: Is there any genetic differentiation among the morphs of A. viridis? Is there any genetic adaptation in this species considering the wide range of habitats it colonizes? And, finally, are stress response genes involved in local adaptation?

Authors

Mallien, C., University of Nice-Sophia Antipolis, France, cedricmallien@gmail.com

Aubin, E., University of Pierre and Marie Curie, France

Christen, R., University of Nice-Sophia Antipolis, France

Furla, P., University of Nice-Sophia Antipolis, France, Paola.FURLA@unice.fr

Forcioli, D., University of Nice-Sophia Antipolis, France, didier.forcioli@unice.fr

Details

Oral presentation

Session #:05
Date: 06/23/2016
Time: 17:45
Location: 313 B

Presentation is given by student: Yes