CarySC, University of Delaware, Lewes, USA, caryc@udel.edu
Hicks, B, J, University of Waikato, Hamilton, New Zealand, b.hicks@waikato,ac,nz
Crawford, N, J, University of Waikato, Hamilton, New Zealand, njc21@waikato.ac.nz
Rueckert, A, , University of Waikato, Hamilton, New Zealand, rueckert-andreas@freenet.de
Coyne, K, J, University of Delaware, Lewes, , kcoyne@udel.edu
 
LOW-LEVEL DETECTION AND ENUMERATION OF DIDYMOSPHENIA GEMINATA USING DNA PROBES (Abstract ID: 274)
The freshwater benthic diatom Didymosphenia geminata is emerging globally as an organism with an extraordinary capacity to impact stream ecosystems by forming persistent blooms of dense mucilaginous mats that can extend for several kilometres. These blooms are associated with large fluctuations in dissolved oxygen levels and shifts in benthic invertebrate communities that significantly impact higher trophic levels. Low numbers, patchy distribution and the existence of morphologically similar diatoms have the potential to confound early detection and monitoring of Didymosphenia. Developments in molecular technology now allow rapid and specific low-level detection of algal species. These techniques involve gene amplification technologies that provide greater sensitivity and specificity of detection and enumeration of algal species compared to microscopy, and are ideally suited for routine monitoring of field samples. We will present a newly developed, highly sensitive (<1cell/ml) quantitative real-time PCR protocol designed specifically for high-throughput detection and enumeration of Didymosphenia. This protocol has been developed along with optimized field collection and preservation procedures designed specifically for diatoms inhabiting river ecosystems. Data recently collected from New Zealand and other global sites will be presented.
 
http://www.biosecurity.govt.nz/didymo
Presentation is (Oral, Poster or No Preference): Oral
Presentation is given by student: No
   
 
           
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