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Sison-Mangus, M. P., University of California Irvine, Irvine, USA, msisonma@uci.edu
Tran, K. ., University of California Irvine, Irvine, USA, kevinnt@uci.edu
Jiang, S. ., University of California Irvine, Irvine, USA, sjiang@uci.edu


Although a diverse suite of epibiotic bacteria is known to be associated with the toxigenic diatom Pseudo-nitzschia , the specific physiological interactions of these epibionts with the diatom host are not fully characterized. Previous literature have dealt on the role of epibiotic bacteria on the toxin production of Pseudo-nitzschia but so far, none have focused on algal growth stimulation or algicidal effect of epibiotic bacteria on this HAB species. Using culture isolation technique and metagenomic approach, we isolated and identified the bacteria that adhere to the surface of several strains of toxigenic Pseudo-nitzschia using 16s rDNA sequencing. We then re-introduced the isolated epibiont bacteria on Pseudo-nitzschia australis and P. pungens cultures to determine their physiological impact on these respective hosts. Interestingly, we found that some bacteria can stimulate the growth of P. australis and P. pungens whereas one epibiont can kill P. australis but not P. pungens . These results suggest that epibiotic bacteria do play an important role on the bloom dynamics of different Pseudo-nitzschia species and may potentially dictate the losers and the winners in a Pseudo-nitzschia bloom event.

Session #:S08
Date: 02-18-2011
Time: 14:30

Presentation is given by student: No