View Abstract

CORAL MICROBIOME RESPONSE TO INORGANIC NUTRIENT ENRICHMENT

The coral holobiont is a complex network of interactions among the cnidarian animal, dinoflagellate mutualist and a diverse consortia of bacterial and archaeal symbionts. Anthropogenic nutrient loading from land-based sources alters the physiology and ecology of corals and coral reefs, but we know little about how the microbial component of the coral holobiont responds. We will present the results of a month-long nutrient chemostat press experiment enriching two widespread coral genera (Pocillopora and Porites) with nitrate and phosphate at 5 levels approximating the range of observed nutrient concentrations (<1 - <10 µM) and ratios (constant 3:1) in reefs of various anthropogenic impact across the Pacific. Corals showed clear changes in core physiological metrics, including buoyant weight, photosynthetic quantum yield, chlorophyll, zooxanthellae and protein content. Changes in the microbiome using 16S-based community analysis will be presented in the context of the nutrient chemistry of the system and the physiology of the corals in a robust statistical framework to differentiate responses among 7 genotypes of each coral species.

Authors

Nelson, C. E., University of Hawai'i, USA, craig.nelson@hawaii.edu

Fox, M. D., Scripps Institution of Oceanography, USA, fox@ucsd.edu

Oliver, T. A., NOAA Coral Reef Ecosystem Program, USA, thomas.oliver@noaa.gov

Remple, K. L., University of Hawai'i, USA, remple@hawaii.edu

Quinlan, Z. A., University of Hawai'i, USA, zquinlan@hawaii.edu

Donahue, M. J., University of Hawai'i, USA

Putnam, H. M., University of Rhode Island, USA, hputnam@uri.edu

Details

Oral presentation

Session #:005
Date: 03/03/2017
Time: 10:30
Location: 306 A

Presentation is given by student: No