FACTORS THAT INFLUENCE THE ASSOCIATION BETWEEN MARINE DIATOM PSEUDO-NITZSCHIA AND THEIR BACTERIAL ASSOCIATES
Every living eukaryotic organism associates with diverse bacterial community called microbiota. In the marine diatom Pseudo-nitzschia, we investigated the factors that shape their association with bacteria. We combined pyrosequencing technique, fitness and domoic acid (DA) assays via transplant experiments to determine if toxin production and co-adaptation plays a role in their association. We also asked if host association with microbiota plays a protective role against algicidal bacteria. We found that the 3 Pseudo-nitzschia species with different DA production have distinct microbial communities. Co-adaptation between algal host and their own microbiota also play a major role as shown by the mutualistic relations to their native host of the bacteria that displays respective algalytic and commensal traits to foreign Pseudo-nitzschia hosts. Bacteria-free Pseudo-nitzschia strains were vulnerable to algalytic attack as compared to their counterpart with microbiota. The elevation or attenuation of DA production by toxic Pseudo-nitzschia after co-culture with native and foreign microbiota is highly dependent on the associated bacterial species. The implications of these results in the restructuring of phytoplankton communities and bottom-up effect on the food web will be discussed.
Sison-Mangus, M., University of California Santa Cruz, USA, email@example.com
Jiang, S., University of California Irvine, USA, firstname.lastname@example.org
Tran, K., University of California Irvine, USA, email@example.com
Kudela, R., University of California Santa Cruz, USA, firstname.lastname@example.org
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