Program and Agenda

Abstract

FACTORS THAT INFLUENCE THE ASSOCIATION BETWEEN MARINE DIATOM PSEUDO-NITZSCHIA AND THEIR BACTERIAL ASSOCIATES

Every living eukaryotic organism associates with diverse bacterial community called microbiota. In the marine diatom Pseudo-nitzschia, we investigated the factors that shape their association with bacteria. We combined pyrosequencing technique, fitness and domoic acid (DA) assays via transplant experiments to determine if toxin production and co-adaptation plays a role in their association. We also asked if host association with microbiota plays a protective role against algicidal bacteria. We found that the 3 Pseudo-nitzschia species with different DA production have distinct microbial communities. Co-adaptation between algal host and their own microbiota also play a major role as shown by the mutualistic relations to their native host of the bacteria that displays respective algalytic and commensal traits to foreign Pseudo-nitzschia hosts. Bacteria-free Pseudo-nitzschia strains were vulnerable to algalytic attack as compared to their counterpart with microbiota. The elevation or attenuation of DA production by toxic Pseudo-nitzschia after co-culture with native and foreign microbiota is highly dependent on the associated bacterial species. The implications of these results in the restructuring of phytoplankton communities and bottom-up effect on the food web will be discussed.

Authors

Sison-Mangus, M., University of California Santa Cruz, USA, msisonma@ucsc.edu

Jiang, S., University of California Irvine, USA, sjiang@uci.edu

Tran, K., University of California Irvine, USA, tran.kevin.nguyen@gmail.com

Kudela, R., University of California Santa Cruz, USA, kudela@ucsc.edu

Details

Oral presentation

Session #:069
Date: 2/25/2014
Time: 08:45
Location: 316 C

Presentation is given by student: No