ECOLOGY AND GENETIC ANALYSIS OF KATABLEPHARIS CRE, A HETEROTROPHIC FLAGELLATE THAT ‘BLOOMS’ IN THE COLUMBIA RIVER ESTUARY DURING THE SPRING
The Pacific Northwest coast is strongly influenced by the Columbia River, which is the second largest river in the U.S. Due to the detritus-driven nature of its estuary, much of the microbial activity is heterotrophic and associated with suspended particles. However, major heterotrophic protists in the estuary are not well characterized. Molecular analysis of small subunit (SSU) rRNA sequence libraries suggests that during April the flagellate Katablepharis CRE is the dominant heterotrophic protist in the Columbia River estuary. Further genetic analysis revealed that its large subunit (LSU) rRNA contains a 332 base pair element that is unique to Katablepharis CRE. This unique sequence element (USE) is GC-rich compared to the rest of the gene and shows no significant similarity to katablepharids in other systems, or any eukaryotes in LSU sequence databases. RT-PCR and FISH with USE-specific probes were performed on estuarine samples collected throughout the year from 2007-2013. This data confirms trends observed in the SSU clone libraries, and suggests Katablepharis CRE could play an important role in the fate of spring phytoplankton organic matter in the Columbia River system.
Kahn, P. L., Oregon Health & Science University, USA, firstname.lastname@example.org
Peterson, T. D., Oregon Health & Science University, USA, email@example.com
Zuber, P., Oregon Health & Science University, USA, Peter Zuber
Time: 16:00 - 18:00
Location: Poster/Exhibit Hall
Presentation is given by student: Yes