Program and Agenda

Abstract

HIGH-RESOLUTION FLOW CYTOMETRY PROFILES OF PROCHLOROCOCCUS AT STATION ALOHA

We performed flow cytometry analysis on 5-meter resolution CTD casts on 5 cruises to station Aloha (central Pacific) from September 2011 to October 2013. All depth profiles are similar and show two distinct population phases and three distinct fluorescence phases. Down to the Deep Chlorophyl Maximum (DCM, at ~ 1% of surface PAR), Prochlorococcus cell abundances are constant at about 10^5/ml and then decrease exponentially below the DCM. When descending, the mean chlorophyll fluorescence of Prochlorococcus increases inversely with light intensity (~0.05-0.06/m). The chlorophyll intensity at the DCM is 50x that of the surface individuals. Cells are also larger showing a Forward Light Scatter signature at the DCM 24x that at the surface. At the DCM and below, Prochlorococcus properties change abruptly. This deep population has 3x higher chlorophyll fluorescence, a characteristic Perpendicular Light Scatter signature and shows a dim but detectable signal in the Phycoerythrin fluorescence channel. These properties remain constant with depth. Below the DCM Prochlorococcus numbers decrease at a rate of ~0.01/m.

ePoster:

Authors

Doggett, J. K., C-MORE/University of Hawaii, USA, doggett@hawaii.edu

van den Engh, G., Advanced Cytometry Group, BD BioSciences, Seattle, WA, USA, ger.engh@me.com

Doblin, M. A., University of Technology, Sydney, Australia, martina.doblin@uts.edu.au

Karl, D. M., C-MORE/University of Hawaii, USA, dkarl@hawaii.edu

Details

Poster presentation

Session #:049
Date: 2/27/2014
Time: 16:00 - 18:00
Location: Poster/Exhibit Hall

Presentation is given by student: No

PosterID: 2458