Program and Agenda

Abstract

INVESTIGATION OF MICROBIAL ADHERENCE AND VIRULENCE FACTORS ASSOCIATED WITH OPEN-OCEAN DERIVED PLASTIC MARINE DEBRIS: VIBRIO BACTERIA AS A MODEL SYSTEM

Plastic Marine Debris (PMD) persists much longer than any natural floating substrate and provides an attachment surface for thin layers of life (termed the Plastisphere). Our previous amplicon sequencing surveys of 16S rRNA genes have shown that Bacteria of the genus Vibrio can comprise a major portion of the Plastisphere – at times nearly 25% of the bacterial community. We adapted a 96-well plate format biofilm quantification assay to survey over 50 Vibrio spp. cultivars for attachment ability to various plastic resins. Some vibrios demonstrated cell density-dependent attachment and/or a preference for plastic resin type. Strikingly, ‘super-colonizer’ vibrios were discovered to form measureable biofilms on plastic in a matter of minutes. In general, biofilm formation phenotypes clustered within Heat Shock Protein 60 (HSP 60) gene phylogenies. We generated a metagenomic dataset of a Vibrio-dominated PMD sample and analyzed it for key adherence and virulence genes. Characterizing Vibrio attachment to plastic will provide a model for PMD colonization and clues to the ecological function of this prevalent group of Plastisphere inhabitants.

Authors

Mincer, T. J., Woods Hole Oceanographic Institution, USA, tmincer@whoi.edu

Guzzetta, V. S., DePauw University, USA, vincentguzzetta_2014@depauw.edu

Slikas, B., Marine Biological Laboratory, bslikas@mbl.edu

Zettler, E. R., Sea Education Association, USA, ezettler@sea.edu

Amaral-Zettler, L. A., Marine Biological Laboratory, USA, amaral@mbl.edu

Details

Oral presentation

Session #:140
Date: 2/24/2014
Time: 11:00
Location: 316 B

Presentation is given by student: No