EXPLORING A NEW APPROACH TO MICRO-SCALE PARTICLE IMAGE VELOCIMETRY (PIV) FOR QUANTIFYING FLOW AROUND FREE-SWIMMING MICROZOOPLANKTON.
The interaction of small organisms with surrounding fluid affects many important processes such as feeding, swimming and escape. However, our current understanding is limited, partially because traditional methods of particle image velocimetry (PIV) become impractical at scales less than a few millimeters and microscope based systems tend to restrict animal motion and can incur ‘wall effects’. Here, we present results from a new approach to small-scale PIV imaging that takes advantage of narrow focal planes present at high magnification. Using a simple, inexpensive, brightfield illumination setup it is possible to obtain a high particle density required for accurate PIV measurements while simultaneously obtaining high resolution kinematics of the organism. Additionally, the thickness of the particle sheet can be adjusted through image post-processing. This is accomplished through the Gaussian distribution of particle contrast about the focal plane to obtain a thin 2D sheet (< 50 µm) required for accurate PIV around small organisms. The ability to observe detailed kinematics simultaneously with fluid motion at small scales will aid our understanding of important processes.
Gemmell, B. J., Marine Science Institute, University of Texas at Austin, USA, email@example.com
Jiang, H., Woods Hole Oceanographic Institution, USA, firstname.lastname@example.org
Buskey, E. J., Marine Science Institute, University of Texas at Austin, USA, email@example.com
Time: 16:00 - 18:00
Location: Poster/Exhibit Hall
Presentation is given by student: No