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Thaler, A. D., Duke University Marine Lab, Beaufort, USA, andrew.david.thaler@gmail.com
Zelnio, K. A., Duke University Marine Lab, Beaufort, USA, kzelnio@gmail.com

AN ASSESSMENT OF MICROSATELLITE MARKERS FOR CONSERVATION AND MANAGEMENT

Conservation and management decisions depend on our ability to define populations and understand how those populations are related. Genetic tools, such as microsatellites, have proven useful for assessing population structure. Microsatellites are highly variable repeat motifs that occur throughout eukaryotic genomes. The behavior of microsatellites markers is poorly understood and there are currently no robust guidelines for deciding how many individual markers are necessary to assess genetic variability. Probability of identity (PID) and probability of sibling identity (PSIB) are metrics that measure the ability of a microsatellite marker set to identify individuals within a sample. We modeled populations under a series of demographic conditions to determine if PID and PSIB could be used to determine if a microsatellite marker set gives an accurate assessment of genetic differentiation. For most cases, a PSIB of < 0.0001 accurately reflected expected pairwise FST, but in cases where a high degree of differentiation exists, no amount of microsatellite markers yielded consistent assessments. We hypothesize that this is due to the independent assortment of microsatellite markers in isolated populations.

Poster presentation

Session #:162
Date: 2/22/2012
Time: 14:00 - 16:00
Location: Poster/Exhibit Hall

Presentation is given by student: Yes

PosterID: A0525