Sessions


029 Aquatic Species Investigation: Environmental DNA applications for aquatic ecosystems

The continued rapid advancement of genomics and associated technologies is opening the door to new techniques for detecting and monitoring biodiversity in any aquatic ecosystem. As all organisms shed DNA into their environment, this environmental DNA (eDNA) signal may now be detected directly from the water column through various molecular detection methods, from quantitative PCR to Next-Generation Sequencing. Researchers worldwide have been developing these revolutionary methods for a variety of applications: environmental bioassessment of aquatic habitats based on molecular analyses of biodiversity, detection and monitoring aquatic invasive species, discovery of pathogens and parasites in aquatic environments, determining sites and populations for conservation of biodiversity, and tracking and uncovering populations of threatened or endangered aquatic species. This session gathers together aquatic scientists pursuing the latest available genomic capabilities to provide new, powerful tools for studying, understanding, and protecting aquatic biodiversity. The session allows researchers to present the current state of eDNA analysis while providing insight into future directions for this work.

Session organizer(s)

Erik Pilgrim, U.S. Environmental Protection Agency
pilgrim.erik@epa.gov

Eric Stein, Southern California Coastal Water Research Project
erics@sccwrp.org

Mehrdad Hajibabaei, University of Guelph/Biodiversity Institute of Ontario
mhajibab@uoguelph.ca



Date: 5/21/2014

Please click on the title of an abstract to view the complete abstract.


Oral Presentations
Location: E 145 - 146

10:00
Lodge, D. M.; CONSERVATION IN A CUP OF WATER: RECIPROCAL FEEDBACKS BETWEEN EDNA TECHNOLOGY AND CONSERVATION MANAGEMENT (Abstract ID:15460) O

10:15
Gibson, J. F.; Shokralla, S.; Baird, D. J.; King, I. W.; Monk, W.; Porter, T. M.; Hajibabaei, M.; IMPROVING THE POWER AND EFFICIENCY OF AQUATIC ECOLOGICAL ASSESSMENT THROUGH THE USE OF DNA METASYSTEMATICS AND NEXT-GENERATION SEQUENCING (Abstract ID:13930) O

10:30
Penalva-Arana, C. D.; Pilgrim, E. M.; Martinson, J. W.; UTILIZING ENVIRONMENTAL BARCODING TO IMPROVE THE ACCURACY AND COMPARABILITY OF ENVIRONMENTAL ASSESSMENTS (Abstract ID:15351) O

10:45
Gillett, D. J.; Stein, E. D.; CAN WE MERGE TRADITIONAL MORPHOLOGICAL AND MOLECULAR TAXONOMIC METHODS FOR USE IN MARINE BENTHIC ASSESSMENT PROGRAMS? (Abstract ID:13477) O

11:00
bouchez, a.; Kermarrec, l.; Rimet, F.; Chardon, c.; chaumeil, p.; frigerio, j. m.; laizet, y.; salin, f.; franc, a.; A NEXT-GENERATION SEQUENCING APPROACH FOR RIVER BIOMONITORING USING BENTHIC DIATOMS (Abstract ID:14368) O

11:15
Goldberg, C. S.; Strickler, K.; Fremier, A. K.; MODELING ENVIRONMENTAL DNA DETECTION OF AQUATIC SPECIES ACROSS SYSTEMS (Abstract ID:14302) O

11:30
Brown, E. A.; Zhan, A.; Flynn, J.; MacIsaac, H.; Cristescu, M. E.; EARLY DETECTION OF AQUATIC INVADERS: CAN NEXT-GENERATION SEQUENCING ACCURATELY DESCRIBE ZOOPLANKTON COMMUNITIES? (Abstract ID:14024) O

11:45
Pawlowski, J.; Esling, P.; Lejzerowicz, F.; Cedhagen, T.; Pochon, X.; Wood, S. A.; Keeley, N.; Wilding, T. A.; ENVIRONMENTAL DNA BARCODING AS A TOOL FOR MONITORING IMPACT ASSOCIATED WITH FISH FARMING ON COMMUNITY OF BENTHIC FORAMINIFERA (Abstract ID:13798) O

13:30
Prichard, C. G.; Blomquist, T. M.; Stepien, C. A.; A NEW NEXT-GENERATION SEQUENCING ASSAY TO IDENTIFY AND QUANTIFY ALL FISH SPECIES FROM AN EDNA WATER SAMPLE (Abstract ID:13990) O

13:45
Pagenkopp Lohan, K. M.; Fleischer, R. C.; Holzer, K. K.; Carney, K. J.; Ruiz, G. M.; AMPLICON-BASED PYROSEQUENCING REVEALS PROTISTAN PARASITE DIVERSITY IN BALLAST WATER (Abstract ID:15190) O

14:00
Wilson, C.; Bronnenhuber, J.; Boothroyd, M.; Serrao, N.; Wozney, K.; Caleigh Smith, C.; Reid, S.; OVERVIEW OF ENVIRONMENTAL DNA TESTING AND APPLICATIONS FOR INVASIVE AND ENDANGERED SPECIES IN ONTARIO (Abstract ID:15537) O

14:15
Simmons, T.; Talbot, S.; Flamme, M.; USING NEXT-GENERATION SEQUENCING OF ENVIRONMENTAL DNA TO ASSESS FISH ASSEMBLAGES IN ALASKA NATIONAL PARKS (Abstract ID:13374) O

14:30
Port, J. A.; Kelly, R. P.; Yamahara, K. M.; Crowder, L. B.; USING ENVIRONMENTAL DNA TO CENSUS FISHES IN MARINE ECOSYSTEMS (Abstract ID:14154) O

14:45
Sformo, T.; Stoeckel, D. M.; Bickham, J. W.; Crawford, J. A.; ENVIRONMENTAL DNA ASSESSMENT OF ARCTIC MARINE FISH FROM THE BEAUFORT SEA OF ALASKA (Abstract ID:15118) O

15:00
Walsh, E. J.; Moody, M. L.; Leung, M. Y.; Gill, T. E.; Hinson, K. I.; ASSESSMENT OF ARIDLAND AQUATIC BIODIVERSITY USING SECOND GENERATION SEQUENCING (Abstract ID:15442) O

15:15
Eaton, W. D.; Shebitz, D. J.; DEVELOPMENT OF FUNGAL FUNCTIONAL GROUPS AND PLANT LIFE FORMS IN ATLANTIC WHITE-CEDAR SWAMPS FROM EARLY RESTORATION AREAS TO OLD GROWTH SWAMPS (Abstract ID:13944) O

16:00
Deiner, K.; Walser, J.; M├Ąchler, E.; Altermatt, F.; CAPTURE AND EXTRACTION METHODS AFFECT BIODIVERSITY DETECTED FROM ENVIRONMENTAL DNA (Abstract ID:14306) O

16:15
Bik, H. M.; PHINCH: AN INTERACTIVE, EXPLORATORY DATA VISUALIZATION FRAMEWORK FOR ENVIRONMENTAL SEQUENCE DATA (Abstract ID:13102) O

16:30
Turner, C. R.; Uy, K. L.; Everhart, R. C.; Jerde, C. L.; Lodge, D. M.; CONCENTRATIONS OF AQUEOUS AND SEDIMENTARY ENVIRONMENTAL DNA (EDNA) REFLECT FISH ABUNDANCE (Abstract ID:14262) O

16:45
Barnes, M. A.; Turner, C. R.; Jerde, C. L.; Lodge, D. M.; ENVIRONMENTAL DNA PARTICLE SIZE DISTRIBUTIONS: IMPLICATIONS FOR COLLECTION AND ANALYSIS (Abstract ID:14775) O

17:00
Shogren, A. J.; Andruszkiewicz, E.; Tank, J. L.; Bolster, D.; Olds, B.; Jerde, C.; PREDICTING THE EFFECT OF HETEROGENEOUS SUBSTRATE ON THE FLUVIAL TRANSPORT OF FISH EDNA USING FLOW-THROUGH COLUMN EXPERIMENTS (Abstract ID:13893) O

17:15
Olds, B. P.; Evans, N.; Jerde, C. L.; Turner, C. R.; Renhaw, M. A.; Uy, K.; Gantz, C.; Li, Y.; Lamberti, G. A.; Lodge, D. M.;EFFECTIVENESS OF TRADITIONAL GEARS VERSUS EDNA IN DETECTING SPECIES RICHNESS OF AQUATIC COMMUNITIES (Abstract ID:14400) O

17:30
Zhang, X.; Yang, J.; Xie, Y.; APPLICATION AND COMPARISON OF THE PCR-DEPENDENT AND PCR-FREE NGS APPROACHES ON BIODIVERSITY ASSESSMENT OF FRESHWATER ZOOPLANKTONS IN TAI LAKE, CHINA (Abstract ID:13133) O


Date: 5/22/2014

Please click on the title of an abstract to view the complete abstract.


Poster Presentations
Location: Poster/Exhibit Hall

130
Klymus, K. E.; Richter, C. A.; Chapman, D. C.; Paukert, C.; QUANTIFICATION OF EDNA SHEDDING RATES FROM INVASIVE BIGHEADED CARPS (Abstract ID:13049) P

131
Martinson, J.; Kostich, M.; Matthews, S.; Penalva-Arana, C.; Pilgrim, E.; ANALYSIS OF THE REPRODUCIBILITY OF METAGENOMIC DATA TO MEASURE AQUATIC BIODIVERSITY (Abstract ID:14033) P

132
Flynn, J.; Brown, E. A.; Chain, F.; MacIsaac , H.; Cristescu, M. E.; CLUSTERING OF PYROSEQUENCE DATA: METHODS TO PRODUCE ACCURATE ESTIMATES OF SPECIES RICHNESS (Abstract ID:14138) P

133
Connor, S. J.; Baird, D. J.; Curry, C.; Gibson, J.; Hajibabaei, M.; Heard, K.; Hussey, B.; King, I.; Monk, W.; Rosolen, S.;APPLYING THE BIOMONITORING 2.0 APPROACH IN TWO CONTRASTING SUB-ARCTIC TRIBUTARIES OF THE SLAVE RIVER, NWT, CANADA. (Abstract ID:14421) P

134
Matthews, S.; Hoffman, J.; Peterson, G.; Barker, J.; Martinson, J.; Pilgrim, E.; IDENTIFICATION OF LAKE SUPERIOR LARVAL FISH ASSEMBLAGES BY NEXT-GENERATION DNA SEQUENCING (Abstract ID:14930) P

135
Pilgrim, E.; Martinson, J.; Matthews, S.; COMPARISON OF ESTIMATES OF AQUATIC BIODIVERSITY USING DIFFERENT GENETIC LOCI (Abstract ID:14937) P