Zepp, R. G., US EPA, Athens GA, USA, zepp.richard@epa.gov
White, E. M., US EPA, Athens GA, USA, white.emily@epa.gov
Molina, M. ., US EPA, Athens GA, USA, molina.marirosa@epa.gov

DIFFERENTIAL EFFECTS OF SOLAR ULTRAVIOLET RADIATION ON CULTURABLE ENTEROCOCCUS FAECALIS AND ENTEROCOCCUS DNA DETERMINED USING REAL-TIME QUANTITATIVE PCR

Biological contamination of aquatic environments by pathogenic microorganisms is often assessed using fecal indicator bacteria such as enterococci. The concentrations of enterococci are commonly determined by culturing techniques, but there has been recent interest in using molecular microbial analysis methods with shorter reporting times. The quantitative polymerase chain reaction (qPCR) applied to detect DNA concentrations of Enterococcus is one rapid technique that shows great promise for evaluating recreational water quality. Very little is known about factors that affect the fate and transport of enterococci culturable and qPCR indicators in freshwater environments. Studies conducted near UV-disinfected effluents of sewage treatment plants indicate that the sensitivity of these two indicators to solar ultraviolet (UV) radiation may be very different. In this paper we compare the effects of solar UV radiation on culturable Enterococcus faecalis with its effects on Enterococcus measured using real-time qPCR. As part of this study, we also report the effects of chromophoric dissolved organic matter (CDOM) and suspended sediments on the light-induced degradation of these indicators. (Although this work was reviewed and approved by EPA, it may not necessarily reflect Agency policy.)

Oral presentation

Presentation is given by student: No
Session #:SS01
Date: Friday, June 13, 2008
Time: 3:15 PM

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