Abstract


CYANOBACTERIA BLOOMS IN LOW-NUTRIENT MAINE LAKES: DEVELOPMENT OF A QPCR ASSAY FOR GLOEOTRICHIA ECHINULATA USING THE ITS REGION (S)

Maine’s lakes contribute to a significant portion of the state’s tourism economy and its public drinking water supply. Across the nation, the water quality of lakes is declining as toxic cyanobacterial blooms become more widespread. The cyanobacterium Gloeotrichia echinulata is increasingly found in lakes throughout New England, including those in our study region (Belgrade Lakes, Maine, USA). Very little is known about the timing, duration, and toxicity of G. echinulata blooms in Maine lakes and even less is known about its genetic diversity. We performed DNA sequencing on isolates of G. echinulata from our study sites for comparative phylogenetic analysis and for development of a quantitative PCR assay that was used for rapid determination of G. echinulata abundance. Time-series samples collected during the summer of 2015 were assayed via qPCR, indicating the presence of G. echinulata cells in almost every lake sample, with peak abundances approaching 200,000 cells per liter. The new qPCR assay will allow us to provide ‘early warning’ of these potentially toxic organisms in our freshwater resources prior to the onset of visually-noticeable blooms.

Authors

Chmiel, R. J., Bigelow Laboratory, USA, rjchmiel@colby.edu

Kim, B. S., Bigelow Laboratory, USA, stkim@colby.edu

Rothschild, H. T., Colby College, USA, htrothsc@colby.edu

Bruesewitz, D. A., Colby College, USA, dabruese@colby.edu

King, D. W., Colby College, USA, dwking@colby.edu

Countway, P. D., Bigelow Laboratory, USA, pcountway@bigelow.org

Details

Poster presentation

Session #:SS04
Date: 06/07/2016
Time: 17:30 - 19:00
Location: Poster/Exhibit Hall

Presentation is given by student: Yes

PosterID: 4