Early Registration Deadline
12 February 2010

Travel Award & Grant Recipients Notified
March 2010

Authors Notified
April 2010

Schedule Posted
January 2010

Lunau, M. ., The Ecosystems Center, MBL, Woods Hole, USA, mlunau@mbl.edu
Erickson, M. ., The Ecosystems Center, MBL, Woods Hole, USA, merickson@mbl.edu
Waldron, M. ., The Ecosystems Center, MBL, Woods Hole, , mwaldronmbl.edu
Ducklow, H. W., The Ecosystems Center, MBL, Woods Hole, , hducklow@mbl.edu

SHIPBOARD, NEAR-REAL-TIME ENUMERATION OF LIVING PHYTOPLANKTON AND BACTERIA ALONG THE WEST ANTARCTIC PENINSULA.

Flow Cytometry (FC) has become a powerful tool in microbial ecology. However, the application is still not widespread in oceanography due to high cost and technical requirements typically limiting use to the laboratory environment. Nevertheless, prompt, direct counting of live microplankton in the field could provide valuable ecological information in near-real time. Moreover, shipboard FC saves time and costs and prevents artifacts by making the preservation, shipping and storage of samples obsolete. In this study we used the Accuri C6 FC on board ship during a research cruise along the West-Antarctic Peninsula in the austral summer 2010. The C6 is an affordable, dual laser FC which allowed excellent identification of pigmented procaryotes, pico- and nano eucaryotes, and DNA-labeled bacteria. The data presented here provide detailed information about the microphytoplankton distribution, their size and granularity, as well as bacterial abundance and membrane activity measurements along a north/south and onshore/offshore gradient from high resolution depth profiles using CTD casts and pump sampling. The decreasing size and increasing resolving power of FCs suggests installation and deployment on moorings, gliders and other autonomous platforms.

Poster presentation

Session #:T20
Date: 06-08-10
Time: 17:30 - 19:30

Presentation is given by student: No