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Tran, K. N., University of California Irvine, Irvine, USA, kevinnt@uci.edu
Jiang, S. ., University of California Irvine, Irvine, USA, sjiang@uci.edu
Sison-Mangus, M. P., University of California Irvine, Irvine, USA,

BACTERIAL DIVERSITY ASSOCIATED WITH TOXIC AND NON-TOXIC PSEUDO-NITZSCHIA SPECIES

Epibiotic bacterial populations are implicated in the toxin production of the toxic Pseudo-nitzschia species. To date, several bacteria from &Alpha-proteobacteria ( Roseabacter, Pseudoalteromonas, Sulfitobacter, Staleya and Paracoccus, Sphingomonas) &Gamma-proteobacteria ( Pseudoalteromonas, Neptunomonas) and Bacteroidetes ( Winogradskyella) have been isolated from the toxin-producing Pseudo-nitzschia multiseries (Kaczmarska et al. 2005, Genbank). In this study, we isolated and identified the epibiotic bacteria associated with the non-toxic P. pungens strain by culture isolation technique and metagenomic approach to determine if their epibiont bacteria is diverse as well. The 16s rDNA was amplified and sequenced from cultured bacteria and from clonal library made from P. pungens metagenomic sample. We found that P. pungens associate with only a few bacteria from &Alpha-proteobacteria group; their 16S sequence identities are similar to cultured and uncultured Thalassobacter and Roseobacter. Our results suggest that bacterial diversity may be a characteristic of toxic Pseudo-nitzschia species.

Session #:S01
Date: 02-14-2011
Time: 09:45

Presentation is given by student: Yes