Abdelzaher, A. ., University of Miami Oceans and, Key Biscayne, FL, USA, aabdelzaher@gmail.com
Wright, M. ., University of Miami Oceans and Human Health Center, Key Biscayne, FL, USA, mary@movingstrength.com
Scott, T. ., University of Miami Oceans and Human Health Center, Key Biscayne, FL, USA, tscott@gator.net
Lucasik, G. ., BCS Laboratories, Gainesville, FL, USA, tscott@gator.net
Solo-Gabriele, H. ., University of Miami Oceans and Human Health Center, Key Biscayne, FL, USA, hmsolo@miami.edu
Bonilla, A. ., University of Miami Oceans and Human Health Center, Key Biscayne, FL, USA, bonillaf@mail.vetmed.ufl.edu
Bonilla, T. ., University of Miami Oceans and Human Health Center, Key Biscayne, FL, USA, bonillaT@mail.vetmed.ufl.edu
Palmer, C. ., University of Miami Oceans and Human Health Center, Key Biscayne, FL, , carpalmer@mail.vetmed.ufl.edu
DUAL LAYER FILTRATION SYSTEM FOR CONCENTRATING FECAL INDICATORS AND PATHOGENS FROM MARINE WATERS
The ability to rapidly and effectively concentrate diverse microbes is an essential component for monitoring water quality at recreational beaches. The purpose of this study was to develop a 90 mm diameter 0.45µm pore size dual membrane system, which can simultaneously concentrate both viruses and either bacteria and/or protozoa. The top PVDF membrane was used to filter bacteria/protozoa by physical straining while the bottom HA membrane retained viruses through adsorption. Results indicated that 73+50% of the test bacteria (E. faecalis) and 82+8% of the test virus (MS2 coliphage) were recovered. Recoveries of Cryptosporidium and Giardia varied between 40 and 70%. The highest recoveries of poliovirus (Polio 1 CHAT) were obtained with a guanidinium hydrochloride elution solution followed by secondary concentration using a commercially available column. Future experiments should focus on measurements utilizing additional bacteria and viruses as well as water with different physical and chemical parameters.
Poster presentation
Presentation is given by student: No
Session #:120
Date: 03-03-2008
Time: 17:30 - 19:30