View Abstract

SUBLETHAL TOXICITY OF THE HARMFUL HAPTOPHYTE PRYMNESIUM PARVUM TO TWO COMMON FISH SPECIES

Anthropogenic activities and climate change have increased the occurrence of harmful algae blooms (HABs). Prymnesium parvum is an invasive haptophyte known as “golden algae” or the “Texas Tide” that forms HABs leading to massive fish kills. Though progress has been made in understanding factors leading to ambient toxicity associated with bloom development and termination, an understanding of how P. parvum affects fish sublethally is unknown. In the present study we aimed: (1) to determine if sublethal biochemical and molecular oxidative stress (OS) responses occur after exposure to P. parvum; (2) to determine if sublethal OS responses are altered by nutrient limitation; and (3) to compare whether OS responses varied between two common fish species, the fathead minnow (Pimephales promelas) and zebrafish (Danio rerio). P. parvum cultures were grown until stationary phase was reached under nutrient sufficient (f/2) and deficient (f/8) conditions following methods previously reported by our group. Initial studies were conducted to identify acute LC50 values for fathead minnow and zebrafish prior to and following exponential growth using standard methods. Sublethal levels of P. parvum cultures were examined with traditional biomarkers of OS (lipid peroxidation, DNA damage, total glutathione), and antioxidant gene activation (changes in nrf2, gclc, gst, sod) in fish larvae. To our knowledge, this study represents the first attempt to understand mechanistic toxicity responses in general and oxidative stress in particular of fish to P. parvum.

Authors

Hill, B. N., Baylor University, USA, Bridgett_Hill@baylor.edu

Corrales, J., Baylor University, USA, Jone_Corrales@baylor.edu

Scott, W. C., Baylor University, USA, Casan_Scott@baylor.edu

Brooks, B. W., Baylor University, USA, Bryan_Brooks@baylor.edu

Details

Poster presentation

Session #:021
Date: 03/03/2017
Time: 11:00 - 12:00
Location: Poster/Exhibit Hall

Presentation is given by student: Yes

PosterID: 225