View Abstract

VERTICAL VARIABILITY IN VIRAL AND HOST ASSEMBLAGES AT STATION ALOHA

Marine bacteriophage shape the ecology and biogeochemistry of microbes through lysis of bacterial hosts, increasing the amount of organic matter that can be respired or recycled in the upper ocean. Although bacteriophage represent the most abundant DNA-containing entities in the oligotrophic oceans, we know little about viral populations across depth in the open ocean. Here, we present an initial analysis of viral metagenomic depth profile from 5 to 175 m at Station ALOHA in the North Pacific Subtropical Gyre. Size-fractionated DNA was extracted, used to prepare TruSeq nano metagenomic libraries, and sequenced using standard Illumina technology. Analyses suggested that i) the relative abundance patterns of general phage groups with depth were similar to those of their putative bacterial hosts, suggesting that viral adsorption and decay operates at scales that limit dispersal beyond host range; ii) unlike bacterial genomes, viral genomes do not display a decrease in GC content with increasing depth, suggesting that viral genomes experience different selective pressures than host genomes; iii) the relative abundance of prophage-like DNA varies with depth, suggesting that certain environments may favor lysogeny. Our observations and analyses provide new insight into the interactions of phage with their environment and hosts in situ. Ongoing studies incorporating fully assembled viral genomes from metagenomes, single cell host genomes, and metagenomic and transcriptomic time series promise to contribute further to our knowledge of viral-host dynamics at Station ALOHA.

Authors

Luo, E., Center for Microbial Oceanography Research and Education, University of Hawaii, USA, elaine.luo@hawaii.edu

Eppley, J. M., Center for Microbial Oceanography Research and Education, University of Hawaii, USA, jmeppley@hawaii.edu

Aylward, F. O., Center for Microbial Oceanography Research and Education, University of Hawaii, USA, frank.o.aylward@gmail.com

Romano, A. R., Center for Microbial Oceanography Research and Education, University of Hawaii, USA, ritchiea@hawaii.edu

DeLong, E. F., Center for Microbial Oceanography Research and Education, University of Hawaii, USA, edelong@hawaii.edu

Details

Poster presentation

Session #:040
Date: 03/01/2017
Time: 15:30 - 16:30
Location: Poster/Exhibit Hall

Presentation is given by student: Yes

PosterID: 354