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SHIFTS IN MICROBIAL COMMUNITY COMPOSITION AND MICROBIAL-MEDIATED PROCESSES WITH CYANOBACTERIAL HARMFUL ALGAL BLOOM FORMATION AND TOXIN OCCURENCE

The occurrence of cyanobacterial harmful algal blooms (HABs) in eutrophic freshwater systems continues to be a human and ecological health problem in the Great Lakes region. The U.S. Geological Survey (USGS) is working in the Western Lake Erie Basin to characterize the spatio-temporal life cycle of HABs, their associated toxins, the genes responsible for cyanotoxin production, and HAB-associated microbial communities in relation to algal bloom formation and toxin production. Amplicon sequencing was used to assess the microbial community of lake sediment prior to bloom formation and the microbial community succession in the water column prior to, during, and after algal blooms. Analysis of the sediment microbial community suggests that Microcystis sp. may overwinter in the sediment and act as a source population for summer HAB formation. Additionally, a seasonal shift in the cyanobacterial community from Synechococcus sp. to a Microcystis sp.-dominated community correlates with algal bloom formation and toxin production. This research was expanded to include shotgun metagenomics sequencing evaluating the functional capabilities of microbial communities during algal bloom formation. Data were analyzed to determine whether there is a correlation between shifts in microbial-mediated processes and biotic and abiotic factors associated with HABs. Understanding how microbial populations change over time with respect to concurrently-collected, water-quality data will help infer which parameters influence changes in the bloom and identify potential triggers for HABs.

Authors

Givens, C. E., USGS Michigan-Ohio Water Science Center, USA, cgivens@usgs.gov

Duris, J. W., USGS Pennsylvania Water Science Center, USA

Stelzer, E. A., USGS Michigan-Ohio Water Science Center, USA

Ecker, C. D., USGS Michigan-Ohio Water Science Center, USA

Larson, J. H., USGS Upper Midwest Environmental Sciences Center, USA

Loftin, K., USGS Kansas Water Science Center, USA

Lenaker, P., USGS Wisconsin Water Science Center, USA

Evan, M. A., USGS Great Lakes Science Center, USA

Details

Oral presentation

Session #:021
Date: 03/02/2017
Time: 14:30
Location: 323 B

Presentation is given by student: No