UNRAVELLING THE ROLE OF NITROGEN, PHOSPHORUS, AND TEMPERATURE IN DRIVING CYANOHAB DIVERSITY USING NEXT GENERATION SEQUENCING
This study aimed to assess the shifts in the toxic cyanobacterial bloom community diversity in response to elevated temperature, nitrogen (N) and phosphorus (P), and their interactions during spatial/temporal monitoring and incubation experiments in Lake Erie (USA) and Mill Pond (Long Island, NY). A bar-coded metagenomic analysis of cyanobacterial-specific 16S rRNA and the microcystin production mcyE genes was conducted using Illumina MiSeq next generation sequencing (NGS) technology. Cyanobacteria biomass significantly increased in response to elevated nutrients and temperature while the abundance of green algae and diatoms decreased. 16S rRNA sequencing revealed high genera diversity in all experiments (37-49 unique Operational Taxonomic Units (OTUs)), with the most abundant genera including Anabaena, Aphanizomenon, Microcystis, and Planktothrix. N was a significant source of variation for non-diazotrophic genera, as Microcystis dominated under high N, lower temperature, and low P conditions, while diazotrophic genera were driven by P and temperature variation, dominating under low N conditions. mcyE sequencing revealed high OTU abundance (112-713 OTUs) but low diversity in microcystin producing genera as all OTUs corresponded to Microcystis or Oscillatoria. Microcystin concentration coincided with the abundance of these sequences. Collectively, this high throughput sequencing revealed the differential roles of N, P, and temperature in promoting different genera of cyanobacteria.
Jankowiak, J. G., Stony Brook University, USA, firstname.lastname@example.org
Gobler, C., Stony Brook University, USA, email@example.com
Hattenrath, T., Stony Brook University, USA, firstname.lastname@example.org
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