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ANALYSIS OF PHOSPHORUS COMPOUNDS BY USING 2D(1H 31P)–NUCLEAR MAGNETIC RESONANCE (NMR) SPECTROSCOPY IN AN OLIGOTROPHIC ENVIRONMENT.

In the current study, we developed a technique to analyze P compounds in suspended particles in an oligotrophic environment. We analyzed them using 2D 1H-31P nuclear magnetic resonance (NMR) spectroscopy. Recently, significant amount of phosphonates compounds, having C-P bond, has been suggested. Furthermore, methane emission from phosphonates are also considered under a strongly P-limited environment (e.g., methylphosphonic acid). The purpose of the current study is to detect several P compounds in an oligotrophic environment. Lake Saiko is an ultraoligotrophic lake, located on the Yamanashi Prefecture in Japan. We used a cartridge filter for collecting the suspended particles efficiently; we filtered through approximately 274 L to collect the sufficient amount of the samples during 130 minutes. The extraction was conducted for 1 hr under water temperature of approximately 60 ˚C and then, P compounds were analyzed by 1H-31P heteronuclear multiple bond correlation (HMBC) spectra. We found that among phosphonates, 2-aminoethylphosphonic acid is included in the suspended particles in Lake Saiko. This compound could also be bioavailable after orthophosphate becomes depleted conditions. The other compounds are nucleotides, such as DNA-P and RNA-P. The use of cartridge filter is useful to collect suspended particles efficiently in the oligotrophic environment, and this method can also be applicable to ocean samples because the P concentration in Lake Saiko is similar to that in ocean.

Authors

Shinohara, R., National Institute for Environmental Studies, Japan, r-shino@nies.go.jp

Iwata, T., University of Yamanashi, Japan, tiwata@yamanashi.ac.jp

Ikarashi, Y., University of Yamanashi, Japan

Sano, T., National Institute for Environmental Studies, Japan

Kohzu, A., National Institute for Environmental Studies, Japan

Details

Poster presentation

Session #:122
Date: 02/27/2017
Time: 15:30 - 16:30
Location: Poster/Exhibit Hall

Presentation is given by student: No

PosterID: 688