Abstract


RECONSTRUCTION OF BALTIC SEA BACTERIOPLANKTON GENOMES FROM TIME-SERIES METAGENOMES UNCOVERS A GLOBAL BRACKISH MICROBIOME

Recent years progress in high-throughput sequencing has enabled us to investigate spatiotemporal patterns of Baltic Sea microbial communities at a taxonomic scale. These studies have provided important insights on the taxonomic composition and e.g. revealed that the Baltic appears to be composed of a mixture of bacterioplankton with closest relatives in freshwater and marine habitats, respectively. Since cultured bacteria rarely represent the dominant members of a natural community we have very little genomic information on the microbial plankton of the Baltic Sea. Here, we have conducted Illumina shotgun metagenomic sequencing on a large number of samples from the Linneaeus Microbial Observatory (LMO) Time-series. By using our novel program CONCOCT for clustering contigs into genomes we were able to reconstruct 30 nearly complete microbial genomes that together constitute ca. 25% of the DNA content in the surface water of the Baltic Proper. The genomes represent members of Actinobacteria, Bacteroidetes, Proteobacteria, Verrucomicrobia, Cyanobacteria and Thaumarchaeaota. Similar to what earlier taxonomy-based studies have shown, these are a mixture of marine and freshwater relatives. However, by comparing with metagenomic data from different aquatic habitats, the high phylogenetic resolution provided by the genomic data shows that the brackish genomes are distinct from marine and freshwater populations, and that a global brackish microbiome exists. Linking the reconstructed genomes to the spatiotemporal distribution of their respective 16S genotypes allows us to infer links between the ecology and the functional potential of these populations.

http://envgen.github.io/

Authors

Hugerth, L. W., KTH Royal Institute of Technology, Sweden, luisa.hugerth@scilifelab.se

Alneberg, J., KTH Royal Institute of Technology, Sweden

Larsson, J., Linnaeus University, Sweden

Pinhassi, J., Linnaeus University

Andersson, A. F., KTH Royal Institute of Technology, Sweden, anders.andersson@scilifelab.se

Details

Oral presentation

Session #:075
Date: 2/26/2015
Time: 15:15
Location: Andalucia 1 (Floor 1)

Presentation is given by student: No