Abstract


GENE FLOW IN THE STREAM CADDISFLY STENOPSYCHE MARMORATA REVEALED BY BOTH NUCLEAR AND MITOCHONDRIAL DNA

We examined genetic structure and introgresson of the stream caddisfly Stenopysche maramorata (Stenopsychidae, Trichoptera) in Japan using 8 microsatellites and mitochondrial cytochrome oxidase I (COI). Previous work using 8 microsatellites found that gene flow ranged from 12-18 km and the species was distinctly separated into upland and lowland (i.e. <250 m a.s.l.) populations. Here we genotyped 7 additional populations (152 individuals) and sequenced mtDNA for all populations (549 individuals). Bayesian clustering analysis based on microsatellites indicated that the altitudinal structure was not as pronounced on other geographical areas. A phylogenetic gene tree of COI resulted in 3 distinct clades within the species: 1 upland, 1 lowland, and 1 altitudinally mixed clade. Combined nuclear-mt analysis revealed introgression in all clades (upland to lowland); however, most introgressed individuals had lowland mt haplotypes which inicates females of S. marmorata disperse from lowland to upland populations.

Authors

YAEGASHI, S., IGB, Germany, yaegashi@igb-berlin.de

WATANABE, K., Ehime university, Japan

MONAGHAN, M. T., Germany

OMURA, T., Tohoku university, Japan

Details

Oral presentation

Session #:007
Date: 2/25/2015
Time: 15:15
Location: Albeniz (Floor -2)

Presentation is given by student: No